apc cy7 anti cd19  (Cell Signaling Technology Inc)

Journal: Antibodies

Article Title: An Engineered Mouse Model That Generates a Diverse Repertoire of Endogenous, High-Affinity Common Light Chain Antibodies

doi: 10.3390/antib13010014

Figure Lengend Snippet: Characterization of B cell-specific immune response upon immunization; Immune repertoire characterization of immunized mice (N = 2 for the antigen A immunized wildtype mice, N = 3 for the antigen A or antigen B immunized cLC mice, respectively). ( a ) Antigen A and antigen B serum antibody titration by ELISA. The error bar represents standard deviation. ( b – d ) Analysis of B cell populations in each experimental group. The tissues from each immunized mice group were collected, stained, and subjected to FACS. ( b ) Percentage of B cell subpopulations in the spleen, as revealed either by surface Kappa and Lambda expression or IgM and IgD expression of single live total B cells (CD19+B220+), or transitional B cells (CD93+B220+) of single live B cells (CD19+), or mature follicular B cells (CD23hiCD21int) and marginal zone B cells (CD23intCD21hi) in total B cells. ( c ) Percentage of B cell subsets in b one marrow. FACS analysis of cells stained for CD43 and B220 expression to identify pro/pre-B cells (B220loCD43+), small pre-B (B220loCD43−), immature/mature (imm/mat B220hiCD43−) B cells. Indicated B cell subsets were pre-gated as single live CD19+ B cells analysis of surface IgM and IgD or surface L chain κ and λ expression for recirculating B cells. Indicated B cell subsets were pre-gated as single live total B cells. ( d ) Analysis of peritoneal B cell subsets as revealed by surface staining of B220 and CD5 in the pre-gated live CD19+ B cells. B cell subsets are defined as follows: B1a (B220−CD5+), B1b (B220−CD5−), B2 (B220+CD5−), and the percentage of each subset was indicated in the graph.

Article Snippet: Cells were then stained with a fluorophore-labeled antibody panel, which includes PerCP-Cy5.5 conjugated anti-mouse IgM (BD Biosciences), PerCP-Cy5.5 conjugated anti-mouse IgD (BD Biosciences), APC-Cy7 conjugated anti-mouse CD19 (BD Biosciences), and a cocktail of phycoerythrin (PE) conjugated antibodies used during sorting as a dump channel: anti-mouse Ly6g (Biolegend, San Diego, CA, USA), PE anti-mouse CD3 (Biolegend), and PE anti-mouse F4/80 (Biolegend) and Alexa647 and Alexa488 ovalbumin (InvivoGen, San Diego, CA, USA).

Techniques: Titration, Enzyme-linked Immunosorbent Assay, Standard Deviation, Staining, Expressing

Journal: The Journal of Experimental Medicine

Article Title: A partial human LCK defect causes a T cell immunodeficiency with intestinal inflammation

doi: 10.1084/jem.20230927

Figure Lengend Snippet: Clinical presentation of P440S LCK patients

Article Snippet: PBMC were stained with anti-CD3-BV510 (#300447; BioLegend), anti-CD8-APC (#555369; BD), anti-CD4-PacBlue (#558116; BD), anti-CD19-APC-Cy7 (#561743; BD), and anti-CD56-PE (#318306; BioLegend).

Techniques:

Journal: The Journal of Experimental Medicine

Article Title: A partial human LCK defect causes a T cell immunodeficiency with intestinal inflammation

doi: 10.1084/jem.20230927

Figure Lengend Snippet: Patient CT scan and TCR repertoire. (A) Bronchiectasis and large bulla in patient P1 CT. (B) Frequency of Vβ usage in patients P1 and P2. (C–E) Frequencies of B cells (CD19 + HLADR + ) (C) memory B cells (CD19 + HLADR + CD27 + ) (D), and memory B cell subsets (isotype switched memory [IgM − IgD − ], IgM memory [IgM + IgD − ], pre-switched [IgM + IgD + ], c-delta class switched [IgM − IgD + ]) (E) from mass cytometry immunophenotyping of age-matched HC and patient PBMCs. Percentages of parent gates are shown.

Article Snippet: PBMC were stained with anti-CD3-BV510 (#300447; BioLegend), anti-CD8-APC (#555369; BD), anti-CD4-PacBlue (#558116; BD), anti-CD19-APC-Cy7 (#561743; BD), and anti-CD56-PE (#318306; BioLegend).

Techniques: Computed Tomography, Mass Cytometry